One of the most important fields of study in medical science is to develop new and improved ways in which to diagnose diseases. The earlier a disease can be diagnosed, the easier it is to treat. Early diagnosis also improves the prognosis. This is why so many scientists concentrate upon the development of new and more efficient tests. One such test that has proven to be invaluable is the enzyme linked immunosorbent assay. With ELISA kits doctors can now diagnose various conditions easily and efficiently.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The importance of being able to detect the presence of antibodies or antigens cannot be overemphasized. Antibodies are proteins that the body produces when a harmful substance in the body is detected. These harmful substances are called antigens. By being able to identify the type of antibody produced by the body, physicians can accurately determine the type of harmful antigens that triggered the production of the antibodies.
The administration of the test is straight forward and painless. A small blood sample is taken from the patient. This sample is then sent to the laboratory where the technician will add the blood sample to an antigen already present in a petri dish. If the blood sample contains antibodies to the antigen, the two substances will bind together. Enzymes are added and the reaction between the antibodies and the antigen is observed.
Diagnosticians can get a lot of information form even the most basic EIA test. However, there are several versions or formats of the test and some are much more complex. The direct EIA, for example, requires that the antigen is screened with a multiwall coat. This makes it more difficult for the antibody to recognize the antigen. A result can only be obtained if the antibody is joined with a specific enzyme.
Then there is the indirect EIA test. In this case the antigen is covered with a polystyrene plate that can only be detected in two distinct stages. The main advantage of this format of the test is that it offers increased sensitivity. Both primary and secondary antibodies can be identified. This entails cost savings and it may even eliminate the need for further tests.
The sandwich EIA test needs pairs of antibodies that are matched in pairs. The first antibody is coated with polystyrene. A second antibody then serves as the detection agent. The purpose of this test is to determine the concentration of the analyte. This test is extremely useful in the diagnosis of latent diseases and it is deemed to be highly accurate.
When all is said and done it is still the early diagnosis of a disease that provides the best chance of recovery and successful treatment. That is why tests such as EIA are so important. They allow doctors to identify the potential or existence of disease at an early stage. This gives the patient a better chance.
This revolutionary technique, also known as EIA, or enzyme immunoassay, is conducted in a laboratory. It is used to determine the concentration of antigens or antibodies in a specific sample. The presence and concentration of antibodies is an important indication of the presence of a variety of medical conditions. The test is extremely popular because it is easy to do and many different tests can be processed at the same time.
The importance of being able to detect the presence of antibodies or antigens cannot be overemphasized. Antibodies are proteins that the body produces when a harmful substance in the body is detected. These harmful substances are called antigens. By being able to identify the type of antibody produced by the body, physicians can accurately determine the type of harmful antigens that triggered the production of the antibodies.
The administration of the test is straight forward and painless. A small blood sample is taken from the patient. This sample is then sent to the laboratory where the technician will add the blood sample to an antigen already present in a petri dish. If the blood sample contains antibodies to the antigen, the two substances will bind together. Enzymes are added and the reaction between the antibodies and the antigen is observed.
Diagnosticians can get a lot of information form even the most basic EIA test. However, there are several versions or formats of the test and some are much more complex. The direct EIA, for example, requires that the antigen is screened with a multiwall coat. This makes it more difficult for the antibody to recognize the antigen. A result can only be obtained if the antibody is joined with a specific enzyme.
Then there is the indirect EIA test. In this case the antigen is covered with a polystyrene plate that can only be detected in two distinct stages. The main advantage of this format of the test is that it offers increased sensitivity. Both primary and secondary antibodies can be identified. This entails cost savings and it may even eliminate the need for further tests.
The sandwich EIA test needs pairs of antibodies that are matched in pairs. The first antibody is coated with polystyrene. A second antibody then serves as the detection agent. The purpose of this test is to determine the concentration of the analyte. This test is extremely useful in the diagnosis of latent diseases and it is deemed to be highly accurate.
When all is said and done it is still the early diagnosis of a disease that provides the best chance of recovery and successful treatment. That is why tests such as EIA are so important. They allow doctors to identify the potential or existence of disease at an early stage. This gives the patient a better chance.
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